Evaluation of Culture Medium for Nisin Production in a
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Evaluation of Culture Medium for Nisin Production in a.

Evaluation of Culture Medium for Nisin Production in a ...

Sep 16, 2008  The best of the tested complex media for nisin production using the PCS biofilm reactor consisted of 4% (w/v) sucrose, 0.02% (w/v) MgSO 4 7H 2 O, and 0.1% (w/v) KH 2 PO 4. Nisin production rate in the biofilm reactor was significantly increased by 3.8-fold (2208 IU/mL) when using the best complex medium tested.

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Evaluation of Culture Medium for Nisin Production in a ...

Request PDF Evaluation of Culture Medium for Nisin Production in a Repeated-Batch Biofilm Reactor In this study, a biofilm reactor with plastic composite support (PCS), made by high ...

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Evaluation of culture medium for nisin production in a ...

Jan 01, 2006  The best of the tested complex media for nisin production using the PCS biofilm reactor consisted of 4% (w/v) sucrose, 0.02% (w/v) MgSO4.7H2O, and 0.1% (w/v) KH2PO4. Nisin production rate in the biofilm reactor was significantly increased by 3.8-fold (2208 IU/mL) when using the best complex medium tested.

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2006a. Evaluation of culture medium for nisin production ...

The best of the tested complex media for nisin production using the PCS biofilm reactor consisted of 4 % (w/v) sucrose, 0.02 % (w/v) MgSO4‚7H2O, and 0.1 % (w/v) KH2PO4. Nisin production rate in the biofilm reactor was significantly increased by 3.8-fold (2208 IU/mL) when using the best complex medium

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Evaluation of Culture Medium for Nisin Production in a ...

Evaluation of Culture Medium for Nisin Production in a Repeated-Batch Biofilm Reactor Pongtharangkul, T.; Demirci, A. download BookSC. Download books for free. Find books

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Culture medium of diluted skimmed milk for the production ...

May 18, 2011  Nisin is a promising alternative to chemical preservatives for use as a natural biopreservative in foods. This bacteriocin has also potential biomedical applications. Lactic acid bacteria are commonly cultivated in expensive standard complex media. We have evaluated the cell growth and nisin production of Lactococcus lactis in a low-cost natural medium consisting of diluted skimmed

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Nisin biotechnological production and application: a ...

Apr 01, 2009  Researchers applied a mixed culture of L. lactis and Saccharomyces cerevisiae for the purpose of stimulating nisin production in a whey-based medium, obtaining value of 85% greater for nisin concentration than that in a pure culture of the bacteria (Liu, Hu, Liu, Chen, 2006).

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ENHANCED NISIN PRODUCTION IN A BIOFILM REACTOR AND ...

evaluated for nisin production using L. lactis NIZO 22186. The high-biomass density of the biofilm reactor contributed to a significantly shorter lag time of nisin production relative to a suspended-cell reactor. The medium evaluation study suggested a complex media for nisin production in the biofilm reactor as: 4% (w/v) sucrose, 0.02% (w/v)

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Development of a low-cost medium for production of nisin ...

10%  Jul 05, 2009  The goal of this project was to develop a lower-cost medium for nisin production, so this bacteriocin could be used in a broader range of industrial fermentation processes. The objectives included: (1) evaluating methods for controlling the inhibitory effect of lactic acid produced during fermentation, and (2) comparing two inexpensive complex media for nisin production.

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[Culture medium optimization and primary kinetics analysis ...

Mar 01, 2001  Response surface methodology was used to optimize a medium for nisin production of Lactococcus lactis. In the first optimization step the influence of sucrose, soybean peptone, yeast extract, potassium dihydrogen phosphate, sodium chloride, and magnesium sulfur on nisin production was evaluated using a fractional factorial design.

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Optimization of nisin production by Lactococcus lactis UQ2 ...

Practical Application: Nisin production was optimized using supplemented whey as alternative culture medium, using a native L. lactis UQ2 strain. Soybean peptone, SW, and subinhibitory amounts of nisin were successfully employed to optimize nisin production by L. lactis UQ2. Dried semipurified nisin showed an activity of 102150 IU/g.

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Nisin biotechnological production and application: a ...

Apr 01, 2009  Researchers applied a mixed culture of L. lactis and Saccharomyces cerevisiae for the purpose of stimulating nisin production in a whey-based medium, obtaining value of 85% greater for nisin concentration than that in a pure culture of the bacteria (Liu, Hu, Liu, Chen, 2006).

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Nisin and pediocin production on mussel-processing waste ...

Average optimization led to a threefold increase in pediocin production (from 322 to 934 BU ml(-1)) and in nisin production (from 32 to 100 BU ml(-1)) when compared with the unsupplemented medium. For this reason, mussel-processing waste warrants further investigation due to its potential use as a c

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Development of a low-cost medium for production of nisin ...

Jul 05, 2009  The goal of this project was to develop a lower-cost medium for nisin production, so this bacteriocin could be used in a broader range of industrial fermentation processes. The objectives included: (1) evaluating methods for controlling the inhibitory effect of lactic acid produced during fermentation, and (2) comparing two inexpensive complex media for nisin production.

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Culture medium of diluted skimmed milk for the production ...

Nisin activity was evaluated using agar diffusion assays. The highest nisin concentration, 49.88 mg/L (3.3 log AU/mL or 1,995.29 AU/mL), was obtained at 16 h of culture, 200 rpm and no airflow (k La=5.29×10 −3). These results show that a cultivation medium composed of diluted skimmed milk supports cell growth to facilitate nisin biosynthesis.

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Enhancement of Nisin Production by Lactococcus lactis ...

May 05, 2016  The objectives of the study were to evaluate the effect of culture medium formulations (ratio of MRS broth and skim milk), effect of nisin concentrations added to culture formulations (0, 0.15, and 0.30 µg/ml), and effect of agitation conditions (0 and 100 rpm) on nisin production by L. lactis BSA.

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(PDF) Effect of Sub-inhibitory Amounts of Nisin and ...

The effect of nisin regulatory system of the quorum-sensing mechanism and mineral salts on the production of nisin A by the native strain Lactococcus lactis UQ2 growing in skim milk was evaluated using a static culture. A 6 × 3 full factorial design

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Optimization of Nisin Production by Lactococcus lactis ...

The production of nisin by batch culture of Lactococcus lactis ATCC 11454 in MRS broth (pH 6.5), as treated in 30 assays, that were set up by a fractional factorial design of two levels (2[4-1 ...

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Production of a nisin Z/pediocin mixture by pH‐controlled ...

Dec 25, 2001  The conditions for high production of nisin Z and pediocin during pH-controlled, mixed-strain batch cultures in a supplemented whey permeate medium with Lactococcus lactis subsp. lactis biovar.diacetylactis UL719, a nisin Z producer strain, and variant T5 of Pediococcus acidilactici UL5, a pediocin-producing strain resistant to high concentrations of nisin, were studied.

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Process and environmental simulation in the validation of ...

The nisin and lactic acid co-production process is divided into three sections (): the core process based on the fermentation stage and the downstream process corresponding to the separation of biomass and products: nisin and lactic acid.Download : Download high-res image (763KB) Download : Download full-size image Fig. 1. Block diagram of nisin production process with lactic acid recovery ...

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Optimization of medium composition for nisin fermentation ...

Nisin is an effective food biopreservative widely used in food industry. However, 1 problem of concern is limited production rate and final nisin concentration. A nisin-producing strain, L. lactis Lac2, a mutant strain with high yield of nisin, was obtained in our laboratory recently. In the present study, a fractional factorial design was applied to investigate the main factors that affect ...

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Optimization of bacteriocin production by Lactobacillus sp ...

Jun 01, 2013  Optimization of culture condition. Subsequent study was carried out to optimize the bacteriocin production at the end of stationary phase (48h) by Lactobacillus sp. MSU3IR and the bacteriocin production was measured in terms of antagonistic activity (AU/ml). The environmental factors, such as pH and temperature has to be optimized for maximum bacteriocin production.

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Publications — Department of Agricultural and Biological ...

Medium evaluation for maximum nisin production by Lactococcus lactis subsp. lactis with or without pH control. Life Sciences Consortium Undergraduate Summer Research Exhibition. Penn State University, University Park, PA.

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Medium Evaluation and Plastic Composite Support Ingredient ...

Evaluation of Culture Medium for Nisin Production in a Repeated-Batch Biofilm Reactor. ... The best of the tested complex media for nisin production using the PCS biofilm reactor consisted of 4% ...

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Optimization of medium composition for nisin fermentation ...

Nisin is an effective food biopreservative widely used in food industry. However, 1 problem of concern is limited production rate and final nisin concentration. A nisin-producing strain, L. lactis Lac2, a mutant strain with high yield of nisin, was obtained in our laboratory recently. In the present study, a fractional factorial design was applied to investigate the main factors that affect ...

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Influence of growth conditions on the nisin production of ...

Apr 01, 2009  The optimal pH for nisin production has previously been found to vary between pH 5.5 and 6.8 depending on the strains of nisin producer and culture media [15, 16]. Nisin Z production with L. lactis IO-1 was optimal at pH 6.0 in xylose media but at pH 5.5 in glucose media . In this study, pH 6.0 was found optimal for nisin production of wild ...

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Production of a nisin Z/pediocin mixture by pH‐controlled ...

Dec 25, 2001  The conditions for high production of nisin Z and pediocin during pH-controlled, mixed-strain batch cultures in a supplemented whey permeate medium with Lactococcus lactis subsp. lactis biovar.diacetylactis UL719, a nisin Z producer strain, and variant T5 of Pediococcus acidilactici UL5, a pediocin-producing strain resistant to high concentrations of nisin, were studied.

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Evaluation of Chitosan-Nisin Coating on Quality ...

Evaluation of Chitosan-Nisin Coating on Quality Characteristic of Fresh Chicken Fillet under ... production in the world. The global meat ... the Nutrient Agar culture medium by using the Pour plate method. Later, the cultures

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Evaluation of culture medium on poly(3-hydroxybutyrate ...

selection, evaluation and improvement of the significant variables of the medium for the production of PHB by Cupriavidus necator ATCC 17697. In order to address the selection of the main factors and optimize the culture medium, a complete factorial experimental design based on the coupled response surface methodology, was presented.

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Evaluation of the antibacterial and sporicidal activity of ...

EOs and nisin against A. acidoterrestris spores, the serial dilution technique was used, in accordance with previously described methodology for vegetative forms. As a negative control, only the BAT medium was used to control for the sterility of the culture medium and plaque. And as a positive control, the

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Nisin-loaded bacterial cellulose: evaluation of its ...

Sep 05, 2020  Standard nisin and bicinchoninic acid kit were purchased from Sigma-Aldrich (Sao Paulo, Brazil). All other reagents were of analytical grade. CB Production and Purification CBs was produced by Gluconacetobacter xylinus ATCC 53582, using 24-well plates with Hestrin Schramm medium. Each well was filled with 1 mL and incubated at 30ºC in static ...

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Optimization of Nisin Production by Lactococcus lactis UQ2 ...

Practical Application: Nisin production was optimized using supplemented whey as alternative culture medium, using a native L. lactis UQ2 strain. Soybean peptone, SW, and subinhibitory amounts of nisin were successfully employed to optimize nisin production by L. lactis UQ2. Dried semipurified nisin showed an activity of 102150 IU/g.

Get Price

Nisin and pediocin production on mussel‐processing waste ...

Jan 21, 2002  Yeast extract and Bacto casitone increased the production of nisin and pediocin, and to a lesser extent biomass synthesis, without significant changes to the final pH of the culture media (all the values were located in the range 472–499 and 381–420 for L. lactis and Ped. acidilactici, respectively).

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Nisin production in a chitin-including continuous ...

The limiting factors in the continuous production of nisin are high amount of biomass loss and low dilution rate application. In this study, a chitin-including continuous nisin fermentation system (CICON-FER) was constructed for high volumetric nisin production using nisin producer L. lactis displaying cell wall chitin-binding domain (ChBD) together with chitin in the reactor. In this respect ...

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Culture medium of diluted skimmed milk for the production ...

Culture medium of diluted skimmed milk for the production of nisin in batch cultivations. Annals of Microbiology, 2012. Adalberto Pessoa. Priscila Mazzola. Júlia Baruque-Ramos. Angela Jozala. Thereza Penna. Adalberto Pessoa.

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Enhanced production of bacterial cellulose by using a ...

Jul 24, 2009  Pongtharangkul T, Demirci A: Evaluation of culture medium for nisin production in a repeated-batch biofilm reactor. Biotechnol Prog 2006, 22: 217-224. 10.1021/bp050295q. Article Google Scholar 27. Kouda T, Yano H, Yoshinaga F: Effect of agitator configuration on bacterial cellulose productivity in aerated and agitated culture.

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Nisin‐producing microorganisms and their implementation in ...

Jun 29, 2015  Growth, acid, and nisin production in different media An inoculum of 5% v/v 45 , from each strain (11 strains were tested) was added into three different culture media: MRS broth medium, first wort (unhopped original beer wort of the Weihenstephan state brewery, 10.5°P) and wort (10.5°P, 6 Bitter Units) according to MEBAK Method 2.9.2.3 and 2 ...

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Optimization of medium composition for the production of ...

Sep 01, 2009  Depending on the indicator strain used, the antibacterial activity was 2‐ to 4‐fold higher in the modified culture medium than in TSB medium under the same conditions. Thin layer chromatography‐bioautography assay showed the presence of three active spots with Rf values of 0.47, 0.7, and 0.82 in TSB medium.

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